A stereomicroscope fluorescence system has a light source, a light guide, a focusing lens assembly, a stereomicroscope with two eyepiece receivers, and two eyepiece assemblies. The light guide is operably attached to receive light from the light source to transmit the light to the focusing lens assembly. The focusing lens assembly includes an excitation filter coated on the focusing lens. The eyepiece assemblies each have a barrier filter and are adapted to be mounted on the eyepiece receivers.