A spectrally encoded imaging device having a light transmission path arrangement which propagates light to illuminate a target object, a light collection path arrangement having a light collection waveguide which propagates a spectrally encoded portion of the light from the target object to a detector which forms an image of the target object accordingly, and a diffractive element which spectrally disperses at least one of the light and the spectrally encoded portion. The light transmission path arrangement and the light collection path arrangement are optically isolated from one another.