Invention Grant
- Patent Title: Method for making polypeptides
- Patent Title (中): 制备多肽的方法
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Application No.: US13510363Application Date: 2010-11-25
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Publication No.: US08815541B2Publication Date: 2014-08-26
- Inventor: Asser Sloth Andersen , Inger Lautrup-Larsen , Per Noergaard
- Applicant: Asser Sloth Andersen , Inger Lautrup-Larsen , Per Noergaard
- Applicant Address: DK Bagsvaerd
- Assignee: Novo Nordisk A/S
- Current Assignee: Novo Nordisk A/S
- Current Assignee Address: DK Bagsvaerd
- Agent Jianjie Hu
- Priority: EP09177095 20091125
- International Application: PCT/EP2010/068178 WO 20101125
- International Announcement: WO2011/064282 WO 20110603
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C07K14/575 ; C12P21/02 ; C12N15/81 ; C12N9/60
Abstract:
The invention provides an improved method for producing polypeptides with a C-terminal glycine in a yeast transformant being characterized in having a non functional KEX1 gene. The method is in particular well suited to produce polypeptides with an aromatic amino acid residue attached to the C-terminal glycine. The yeast strain may have further non-functional protease genes selected from PEP4, YPS1, MKCI, YPS3, YPS5, YPS6, YPS7, PRB1, STE13 and KEX2.
Public/Granted literature
- US20120282653A1 METHOD FOR MAKING POLYPEPTIDES Public/Granted day:2012-11-08
Information query
