A hemostasis assembly having a selectively expandable hemostasis member can be used with a medical device and in a method for delivering a closure element through tissue and into an opening formed in, or adjacent to, a wall of a blood vessel or other body lumen of any size while maintaining or improving hemostasis. The medical device and method of use utilizes such an expandable hemostasis member to maintain or improve hemostasis throughout a medical procedure for closing and/or sealing openings through tissues and/or blood vessels.