Invention Grant
- Patent Title: Thermostable luciferases and methods of production
- Patent Title (中): 热稳定性荧光素酶和生产方法
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Application No.: US13213457Application Date: 2011-08-19
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Publication No.: US08822170B2Publication Date: 2014-09-02
- Inventor: Keith V. Wood , Monika G. Wood , Mary P. Hall
- Applicant: Keith V. Wood , Monika G. Wood , Mary P. Hall
- Applicant Address: US WI Madison
- Assignee: Promega Corporation
- Current Assignee: Promega Corporation
- Current Assignee Address: US WI Madison
- Agency: Michael Best & Friedrich LLP
- Main IPC: C12N9/02
- IPC: C12N9/02 ; C12N11/00 ; C12Q1/66
Abstract:
Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
Public/Granted literature
- US20120009647A1 THERMOSTABLE LUCIFERASES AND METHODS OF PRODUCTION Public/Granted day:2012-01-12
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