Invention Grant
US08822180B2 Method of synthesizing a suppressor tRNA, DNA construct and use thereof for producing a non-natural amino acid-incorporated protein
有权
合成抑制子tRNA的方法，DNA构建体及其在制备非天然氨基酸掺入蛋白质中的应用
- Patent Title: Method of synthesizing a suppressor tRNA, DNA construct and use thereof for producing a non-natural amino acid-incorporated protein
- Patent Title (中): 合成抑制子tRNA的方法，DNA构建体及其在制备非天然氨基酸掺入蛋白质中的应用
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Application No.: US13460773Application Date: 2012-04-30
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Publication No.: US08822180B2Publication Date: 2014-09-02
- Inventor: Shigeyuki Yokoyama , Kensaku Sakamoto , Nobumasa Hino , Takahito Mukai , Takatsugu Kobayashi
- Applicant: Shigeyuki Yokoyama , Kensaku Sakamoto , Nobumasa Hino , Takahito Mukai , Takatsugu Kobayashi
- Applicant Address: JP Saitama
- Assignee: Riken
- Current Assignee: Riken
- Current Assignee Address: JP Saitama
- Agency: Birch Stewart Kolasch & Birch, LLP
- Priority: JP2006-045788 20060222
- Main IPC: C12N15/11
- IPC: C12N15/11 ; C12N15/09 ; C12N15/00
Abstract:
There are provided a DNA construct comprising a suppressor tRNA gene of a non-eukaryote containing no internal promoter functioning in a eukaryotic cell, and a eukaryotic or bacteriophage promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing protein incorporating a non-natural amino acid by using the same.
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