Invention Grant
- Patent Title: High-resolution fluorescence microscopy
- Patent Title (中): 高分辨率荧光显微镜
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Application No.: US13852873Application Date: 2013-03-28
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Publication No.: US08822956B2Publication Date: 2014-09-02
- Inventor: Thomas Kalkbrenner , Ingo Kleppe
- Applicant: Carl Zeiss Microscopy GmbH
- Applicant Address: DE Jena
- Assignee: Carl Zeiss Microscopy GmbH
- Current Assignee: Carl Zeiss Microscopy GmbH
- Current Assignee Address: DE Jena
- Agency: Patterson Thuente Pedersen, P.A.
- Priority: DE102012205032 20120328
- Main IPC: G01N21/64
- IPC: G01N21/64

Abstract:
A microscopy method for producing a high-resolution image of a 2-dimensional sample. The method includes exciting statistically blinking fluorophores in a sample by irradiating the sample with illumination radiation, repeatedly imaging the sample onto a spatially resolving detector in an image field that covers only a part of the sample to thereby obtain a frame sequence, generating an image from the frame sequence, the image having a spatial resolution increased beyond the optical resolution limit using a cumulant function, moving the position of the image field on the sample at least once and repeating the imaging and generating steps to obtain one image for each position of the image field, and combining the resultantly obtained images to form a complete image of the sample.
Public/Granted literature
- US20130256563A1 HIGH-RESOLUTION FLUORESCENCE MICROSCOPY Public/Granted day:2013-10-03
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