Invention Grant
- Patent Title: Multiplex amplification methods
- Patent Title (中): 多重扩增方法
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Application No.: US13118363Application Date: 2011-05-27
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Publication No.: US08828688B2Publication Date: 2014-09-09
- Inventor: Eugeni A. Namsaraev
- Applicant: Eugeni A. Namsaraev
- Applicant Address: US CA Santa Clara
- Assignee: Affymetrix, Inc.
- Current Assignee: Affymetrix, Inc.
- Current Assignee Address: US CA Santa Clara
- Agency: Banner & Witcoff, Ltd.
- Main IPC: C40B50/06
- IPC: C40B50/06 ; C12Q1/68

Abstract:
Compositions and methods for amplifying selected polynucleotides, including DNA and RNA, particularly in multiplex amplification reactions using common primers amplification. Generally, methods of the invention employ multiple steps such as template-specific hybridization, a linear amplification, partial degradation of nucleic acid, and ligation. At the end of the process the sequences of selected polynucleotides are flanked by the common sequences which can be used for exponential amplification using common primers. In some aspects the polynucleotides are associated with a barcode and the presence of the barcode is detected to measure the amount of the polynucleotide.
Public/Granted literature
- US20110294689A1 Multiplex Amplification Methods Public/Granted day:2011-12-01
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