Invention Grant
- Patent Title: Engineered amine dehydrogenases and methods of use thereof
- Patent Title (中): 工程胺脱氢酶及其使用方法
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Application No.: US13895173Application Date: 2013-05-15
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Publication No.: US08835136B2Publication Date: 2014-09-16
- Inventor: Andreas Sebastain Bommarius , Michael Justin Abrahamson , Bettina Bommarius
- Applicant: Georgia Tech Research Corporation
- Applicant Address: US GA Atlanta
- Assignee: Georgia Tech Research Corporation
- Current Assignee: Georgia Tech Research Corporation
- Current Assignee Address: US GA Atlanta
- Agency: Pabst Patent Group LLP
- Main IPC: C12N9/06
- IPC: C12N9/06 ; C12N9/96 ; G01N33/535

Abstract:
Non-naturally occurring amine dehydrogenases (AmDH) and methods of use thereof the produce chiral amines are disclosed. The AmDH are variants of amino acid dehydrogenases. AmDH based on phenylalanine, leucine, and valine scaffolds are provided. The AmDH typically have one, two, three, four, or more amino acid alterations relative to the scaffold. The alterations to the scaffold result in an enzyme that accepts the analogous ketone, such as methyl isobutyl ketone (MIBK), instead of the wild-type α-keto acid. Chimeric AmDH are also disclosed. The chimeras are fusion proteins that include a substrate binding domain from a first AmDH and a cofactor binding domain from a second AmDH. In a preferred embodiment, one of the domains is from a PheDH-based AmDH and one of the domains is from a LeuDH-based AmDH.
Public/Granted literature
- US20130309734A1 Engineered Amine Dehydrogenases and Methods of Use Thereof Public/Granted day:2013-11-21
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