A method for estimation of hematocrit of a sample having an analyte includes: bringing the sample into contact with a sensor strip including a reference electrode coated with a reference layer nonreactive to the analyte, and a working electrode coated with a working layer including a biorecognition element reactive to the analyte; applying a voltage between the reference and working electrodes, and recording information of a reaction current flowing through the sample within a time period of not greater than 5 seconds from beginning of application of the voltage; and estimating the hematocrit of the sample by analyzing the recorded information using a predetermined current-hematocrit function.