Invention Grant
US08841106B2 Puro-DHFR quadrifunctional marker and its use in protein production
有权
Puro-DHFR四官能标记及其在蛋白质生产中的应用
- Patent Title: Puro-DHFR quadrifunctional marker and its use in protein production
- Patent Title (中): Puro-DHFR四官能标记及其在蛋白质生产中的应用
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Application No.: US13742495Application Date: 2013-01-16
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Publication No.: US08841106B2Publication Date: 2014-09-23
- Inventor: Michel Kobr , Philippe Dupraz
- Applicant: Merck Serono SA
- Applicant Address: CH Coinsins, Vaud
- Assignee: Merck Serono SA
- Current Assignee: Merck Serono SA
- Current Assignee Address: CH Coinsins, Vaud
- Agency: Saliwanchik, Lloyd & Eisenschenk
- Priority: EP07109829 20070607
- Main IPC: C12N9/00
- IPC: C12N9/00 ; C12N15/00 ; G01N33/74 ; C12N9/96
Abstract:
This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).
Public/Granted literature
- US20130171714A1 PURO-DHFR QUADRIFUNCTIONAL MARKER AND ITS USE IN PROTEIN PRODUCTION Public/Granted day:2013-07-04
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