中科微点-全球专利检索

  1. Login
  2. Sign Up
Invention Grant
US08841419B2 Hybridoma cell line 10G4 and a monoclonal antibody against the total of aflatoxin B1, B2, G1 and G2 有权
杂交瘤细胞系10G4和抗黄曲霉毒素B1，B2，G1和G2总量的单克隆抗体

Hybridoma cell line 10G4 and a monoclonal antibody against the total of aflatoxin B1, B2, G1 and G2
Abstract:
Hybridoma cell line 10G4 and monoclonal antibody against total aflatoxins produced by the hybridoma cell line 10G4. The hybridoma cell line 10G4 is used to produce the monoclonal antibody that binds specifically total aflatoxin B1, B2, G1 and G2. The titer of the mouse ascites antibody produced by the 10G4 treated mouse is determined through non-competitive enzyme-linked immunosorbent assay and the titer can reach up to 5.12×105. The monoclonal antibody against total aflatoxin B1, B2, G1 and G2 are used for better identification of aflatoxin B1, B2, G1 and G2 with good consistency. The 50% inhibitory concentrations (IC50) of the antibody against aflatoxin B1, B2, G1 and G2 are 2.09 ng/mL, 2.23 ng/mL, 2.19 ng/mL and 3.21 ng/mL respectively. The range of cross reaction rate for aflatoxin B1, B2, G1 and G2 is about 65.2%-100%. The antibody is used for quantitative measurement of total aflatoxin B1, B2, G1 and G2.
Abstract(Chinese):

杂交瘤细胞系10G4和针对由杂交瘤细胞系10G4产生的总黄曲霉毒素的单克隆抗体。 杂交瘤细胞系10G4用于产生特异性结合总黄曲霉毒素B1，B2，G1和G2的单克隆抗体。 通过非竞争性酶联免疫吸附试验测定10G4处理小鼠产生的小鼠腹水抗体滴度，滴度可达5.12×105。 抗黄曲霉毒素B1，B2，G1，G2的单克隆抗体用于更好地鉴定黄曲霉毒素B1，B2，G1和G2，具有良好的一致性。 抗体对黄曲霉毒素B1，B2，G1和G2的50％抑制浓度（IC50）分别为2.09 ng / mL，2.23 ng / mL，2.19 ng / mL和3.21 ng / mL。 黄曲霉毒素B1，B2，G1和G2的交叉反应率范围约为65.2％-100％。 该抗体用于总黄曲霉毒素B1，B2，G1和G2的定量测定。

Public/Granted literature
Information query
Espacenet
Patent Agency Ranking
0/0