Invention Grant
US08846349B2 Sequences and their use for detection and characterization of E. coli O157:H7
有权
序列及其用于检测和表征大肠杆菌O157：H7的用途
- Patent Title: Sequences and their use for detection and characterization of E. coli O157:H7
- Patent Title (中): 序列及其用于检测和表征大肠杆菌O157：H7的用途
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Application No.: US12839837Application Date: 2010-07-20
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Publication No.: US08846349B2Publication Date: 2014-09-30
- Inventor: Frank R. Burns
- Applicant: Frank R. Burns
- Applicant Address: US DE Wilmington
- Assignee: E.I. du Pont de Nemours and Company
- Current Assignee: E.I. du Pont de Nemours and Company
- Current Assignee Address: US DE Wilmington
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C07H21/02 ; C07H21/04
Abstract:
This invention relates to a rapid method for detection and characterization of Escherichia coli bacteria serotype O157:H7 based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect E. coli O157:H7 in a food or water sample, such as a beef enrichment. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.
Public/Granted literature
- US20110020823A1 SEQUENCES AND THEIR USE FOR DETECTION AND CHARACTERIZATION OF E. COLI O157:H7 Public/Granted day:2011-01-27
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