Invention Grant
- Patent Title: Method for qualitative and quantitative detection of common wheat
- Patent Title (中): 普通小麦定性和定量检测方法
-
Application No.: US13518298Application Date: 2010-12-17
-
Publication No.: US08865433B2Publication Date: 2014-10-21
- Inventor: Kazumi Kitta , Satoshi Furui , Junichi Mano , Yasuyuki Matsuoka , Shinichiro Arami , Megumi Sato , Hiroyuki Haraguchi , Youichi Kurimoto , Shinjiro Imai , Keiko Tanaka
- Applicant: Kazumi Kitta , Satoshi Furui , Junichi Mano , Yasuyuki Matsuoka , Shinichiro Arami , Megumi Sato , Hiroyuki Haraguchi , Youichi Kurimoto , Shinjiro Imai , Keiko Tanaka
- Applicant Address: JP Tokyo JP Tokyo
- Assignee: Nippon Flour Mills Co., Ltd,Nisshin Seifun Group Inc
- Current Assignee: Nippon Flour Mills Co., Ltd,Nisshin Seifun Group Inc
- Current Assignee Address: JP Tokyo JP Tokyo
- Agency: Sughrue Mion, PLLC
- Priority: JP2009-289340 20091221
- International Application: PCT/JP2010/072806 WO 20101217
- International Announcement: WO2011/078093 WO 20110630
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68

Abstract:
Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.
Public/Granted literature
- US20120264128A1 METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF COMMON WHEAT Public/Granted day:2012-10-18
Information query