A fiber optic coiled tubing assembly of multiple segments and coupling mechanism therefor. The assembly may be assembled from multiple coiled tubing segments which are pre-loaded with fiber optic line. Thus, the coupling mechanism may be employed for physical coupling of the coiled tubing segments as well as communicative coupling of the lines of the separate segments to one another. As such, pumping of a single fiber optic line through the coiled tubing assembly following coupling of the segments may be avoided. This may be of particular benefit for offshore operations where the joining of multiple coiled tubing segments is likely due to crane load capacity limitations and where such pumping may consume vast amounts of time.