Blood vessel preservation technology is disclosed wherein harvested blood vessels are attached to stationary attachment fixtures at one end, and to a non-stationary (freely extending) fixture at the other end. The vessel and fixtures are placed within a container. Thereafter, a stream of pulsatile blood is directed through the fixtures and the lumen of the vessel causing the vessel to assume full natural length and aligning the vessel with the container. The pulsatile blood then completely bathes the outside surface of the vessel before being re-circulated. The methods disclosed include those wherein the patient's own circulatory system is part of the circulatory path flowing through the vessel and methods in which the circulatory path is outside and apart from the patient. A pulsatile pump, blood heater and pressure regulator may all be utilized in preferred embodiments of the disclosed methods and devices.