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US08877434B2 Method and system of detecting dioxin-like compounds 有权
检测二恶英类化合物的方法和系统

  • Patent Title: Method and system of detecting dioxin-like compounds
  • Patent Title (中): 检测二恶英类化合物的方法和系统
  • Application No.: US13451498
    Application Date: 2012-04-19
  • Publication No.: US08877434B2
    Publication Date: 2014-11-04
  • Inventor: Hsinyu Lee
  • Applicant: Hsinyu Lee
  • Agency: WPAT, P.C.
  • Agent Anthony King; Kay Yang
  • Main IPC: C12Q1/00
  • IPC: C12Q1/00 ;  C12Q1/66
Method and system of detecting dioxin-like compounds
Abstract:
The invention relates to a method or a cell free system of detecting dioxin-like compounds in a test sample using a whole cell lysate derived from a cell transfected to express a fusion protein comprising an AHR fused to a reporter peptide. The method in combination with bioluminescence resonance energy transfer (BRET) technique is also provided so as to improve the sensitivity.
Abstract(Chinese):

本发明涉及使用衍生自转染细胞的全细胞裂解物来检测测试样品中的二恶英样化合物的方法或无细胞系统，以表达包含与报道肽融合的AHR的融合蛋白。 还提供了结合生物发光共振能量转移（BRET）技术的方法，以提高灵敏度。

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