Invention Grant
- Patent Title: Process for purifying cyclolipopeptide compounds or the salts thereof
- Patent Title (中): 环肽肽化合物或其盐的纯化方法
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Application No.: US13877141Application Date: 2011-09-27
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Publication No.: US08877777B2Publication Date: 2014-11-04
- Inventor: Zhaoli Zhang , Shidong Liu , Zhonghao Zhuo , Xiaoming Ji
- Applicant: Zhaoli Zhang , Shidong Liu , Zhonghao Zhuo , Xiaoming Ji
- Applicant Address: CN Shanghai
- Assignee: Shanghai Techwell Biopharmaceutical Co., Ltd.
- Current Assignee: Shanghai Techwell Biopharmaceutical Co., Ltd.
- Current Assignee Address: CN Shanghai
- Agency: Weaver Austin Villeneuve & Sampson LLP
- Agent Tom Hunter
- Priority: CN201010298554 20100930
- International Application: PCT/CN2011/080227 WO 20110927
- International Announcement: WO2012/041220 WO 20120405
- Main IPC: A61K38/12
- IPC: A61K38/12 ; C07K5/00 ; C07K7/00 ; C07K16/00 ; C07K17/00 ; C07K7/56

Abstract:
A method for purifying a cyclic lipopeptide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved.
Public/Granted literature
- US20130296529A1 PROCESS FOR PURIFYING CYCLOLIPOPEPTIDE COMPOUNDS OR THE SALTS THEREOF Public/Granted day:2013-11-07
Information query
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