A technique performs an authentication operation using pulse and facial data from a user. The technique involves obtaining current pulse data from a user, and performing a comparison between the current pulse data from the user and expected pulse data for the user. The technique further involves generating an authentication result based on the comparison between the current pulse data and the expected pulse data. The authentication result may control user access to a computerized resource. Since such a technique uses pulse data, a perpetrator cannot simply submit a static image of a subject's face to circumvent the authentication process. In some arrangements, the technique involves obtaining videos of human faces and deriving cardiac pulse rates from the videos. For such arrangements, a standard webcam can be used to capture the videos. Moreover, such techniques are capable of factoring in circadian rhythms and/or aging adjustments to detect and thwart video replay attacks.