A microfluidic device provided with a micro-channel structure capable of easily and positively providing therein micro-droplet having various dilution ratios. A micro-channel structure provided in a substrate (2) has a first mixing unit (11) and a second mixing unit (21) connected to the downstream side of the first mixing unit (11), with each mixing unit (11, 21) having first through third micro-channels. One end of a first weighing unit (11d) consisting of a micro-channel having a capacity equivalent to the volume of a specified-amount first micro-droplet is opened to a first micro-channel (11a), and the other end is opened to a merging unit (12a) provided on a second micro-channel (12). One end of a second weighing unit (13d) consisting of a micro-channel having a capacity equivalent to the volume of a specified-amount second micro-droplet is connected to a third micro-channel (13), and the other end is opened to the merging unit (12a). Any one of the first through third outlet ports of the first mixing unit is connected with the first or the third inlet port of the second mixing unit (21).