An image sensor may be provided that includes an image pixel array, analog column circuitry and digital column circuitry. The digital column circuitry may extract a systematic analog signal offset from data received from the analog column circuitry. The digital column circuitry may generate analog signal offset correction values based on the systematic analog signal offsets and provide the analog signal offset correction values to the analog column circuitry. The analog column circuitry may remove signal offsets from subsequently read out image data from the image pixel array using the analog signal offset correction values provided by the digital column circuitry. The image pixel array may include image pixels having color filters of various colors. The digital column circuitry may generate analog signal offset correction values corresponding to each of the various colors.