A sequence of three-dimensional thermal infrared images of the face or other area of the body is analyzed to extract time-varying maps which link skin surface temperature details to the locations of anatomical structures. Frame-to-frame quantitative variations in temperature and position of anatomical nodes of the body maps are determined for the sequence and compared to reference profiles to produce estimates of physiological condition and psychological state. The magnitude, latency, and duration of such variations at particular anatomical locations resulting from selected controlled stimuli have been determined to be strongly correlated with particular psychological states.