An electrocardiogram (ECG) signal sensing module is provide. The ECG signal sensing module includes at least one touchpad button, for producing a press signal when being pressed; at least one sensing electrode, mounted on the at least one touchpad button and moving in unison with the at least one touchpad button, for measuring the voltage on the portion thereof touched by a user; an electrocardiogram signal processor, coupled to the at least one sensing electrode, for processing the voltage on the at least one sensing electrode and producing an ECG signal; and a transmission interface, coupled to the at least one touchpad button and the electrocardiogram signal processor, for outputting the press signal and the ECG signal to a computer.