Disclosed is a multi-dimensional gas chromatography chip with a modulator. The multi-dimensional gas chromatography chip includes a chip body including first to third substrates; first micro channel pattern parts including first micro channels; second micro channel pattern parts including second micro channels; a gas inlet to supply a mobile phase; a gas outlet to discharge the mobile phase; position alignment markers formed on one surface of the first substrate, both surfaces of the second substrates and one surface of the third substrate; a first stationary phase spin-coated between the first and second substrates and a second stationary phase spin-coated between the second and third substrates; and a modulator provided in a region serving as an inlet side of the first micro channel pattern parts as well as an outlet side of the second micro channel pattern parts.