Invention Grant
- Patent Title: Pyrococcus furiosus strains and methods of using same
- Patent Title (中): 激烈火球菌菌株及其使用方法
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Application No.: US13248377Application Date: 2011-09-29
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Publication No.: US08927254B2Publication Date: 2015-01-06
- Inventor: Gina L. Lipscomb , Joel Andrew Farkas , Michael W. W. Adams , Janet Westpheling
- Applicant: Gina L. Lipscomb , Joel Andrew Farkas , Michael W. W. Adams , Janet Westpheling
- Applicant Address: US GA Athens
- Assignee: University of Georgia Research Foundation, Inc.
- Current Assignee: University of Georgia Research Foundation, Inc.
- Current Assignee Address: US GA Athens
- Agency: Mueting, Raasch & Gebhardt, P.A.
- Main IPC: C12N1/15
- IPC: C12N1/15 ; C12N1/00 ; C12N15/74

Abstract:
Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 103 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.
Public/Granted literature
- US20120135411A1 PYROCOCCUS FURIOSUS STRAINS AND METHODS OF USING SAME Public/Granted day:2012-05-31
Information query
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