Invention Grant
- Patent Title: Derivatization of PNGase F released glycans on an HPLC chip
- Patent Title (中): PNGase F的衍生在HPLC芯片上释放聚糖
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Application No.: US13953105Application Date: 2013-07-29
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Publication No.: US08927263B2Publication Date: 2015-01-06
- Inventor: Brian Phillip Smart , Magdalena Anna Ostrowski , Gregory Staples
- Applicant: Agilent Technologies, Inc.
- Applicant Address: US CA Santa Clara
- Assignee: Agilent Technologies, Inc.
- Current Assignee: Agilent Technologies, Inc.
- Current Assignee Address: US CA Santa Clara
- Main IPC: C12Q1/34
- IPC: C12Q1/34 ; G01N30/60 ; G01N30/88
Abstract:
A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.
Public/Granted literature
- US20140038215A1 DERIVATIZATION OF PNGASE F RELEASED GLYCANS ON AN HPLC CHIP Public/Granted day:2014-02-06
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