Methods employing an electric field for disinfection of a root canal preparation during an endodontic procedure. A monopolar electrode probe sized and configured for insertion into a root canal of a tooth is inserted within the root canal preparation. A ground electrode is electrically connected to the patient to complete the circuit with the monopolar electrode probe. An electrical voltage is applied between the probe and ground, resulting in virtually no current flow between the electrodes, but rather the generation of an electric field within the root canal and adjacent dental tissues. The electric field kills microbes within the root canal system of the tooth without significant heating or damage to surrounding dental tissue.