Invention Grant
US08932828B2 Method for preparing recombinant granulocyte colony stimulating factor cysteine muteins
有权
重组粒细胞集落刺激因子半胱氨酸突变蛋白的制备方法
- Patent Title: Method for preparing recombinant granulocyte colony stimulating factor cysteine muteins
- Patent Title (中): 重组粒细胞集落刺激因子半胱氨酸突变蛋白的制备方法
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Application No.: US12893764Application Date: 2010-09-29
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Publication No.: US08932828B2Publication Date: 2015-01-13
- Inventor: Mary S. Rosendahl , George N. Cox , Daniel H. Doherty
- Applicant: Mary S. Rosendahl , George N. Cox , Daniel H. Doherty
- Applicant Address: US CO Boulder
- Assignee: Bolder Biotechnology, Inc.
- Current Assignee: Bolder Biotechnology, Inc.
- Current Assignee Address: US CO Boulder
- Agency: Sheridan Ross P.C.
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C07K1/00 ; C07K1/14 ; C07K1/16 ; C07K2/00 ; C07K14/515 ; C07K1/113 ; C07K14/505 ; C07K14/56 ; C07K14/535 ; C07K14/52 ; C07K14/61 ; A61K47/48 ; C07K14/78
Abstract:
The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.
Public/Granted literature
- US20110189124A1 METHODS FOR REFOLDING PROTEINS CONTAINING FREE CYSTEINE RESIDUES Public/Granted day:2011-08-04
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