This invention discloses a LED mixed lighting for tissue culture of orchids. Due to limitation of technology, currently it lacks of LED lighting directly used for orchids, especially for tissue culture of dendrobium candidum, ornamental dendrobium and phalaenopsis. This inventions adopts a technical proposal as follows: a LED fixed lighting for tissue culture of orchids is characterized in that said many red and blue LEDs will constitute several units; each unit has 3 blue LEDs and there are 8 red LEDs in elliptical or rounded patterns surrounding said 3 blue LEDs, and said 3 blue LEDs are gathered in triangular pattern, and said 8 red LEDs are separated at interval. This invention makes special arrangement of lamps and realizes adjustment of light quality and light period by turning on or turning off red LEDs and blue LEDs, and by this way, a LED mixed light which is distributed evenly and suitable for tissue culture of orchids.