A tissue closing device includes an elongate arrangement device of which a distal end portion can penetrate an opening penetrating an living tissue membrane and which has a handling portion on the proximal side, and a clip which is detachably retained at a distal end portion of the arrangement device and which closes the opening. The arrangement device includes a retaining member for detachably retaining the clip and pulling the clip in the proximal direction, a cover tube, and a fixed tube. The handling portion has a first elastic member. When a restriction for maintaining the coil spring in a deformed state is canceled, the thread is moved to the proximal direction by the restoring force of the coil spring in the condition where the clip is locked to a distal end portion of the fixed tube, and the clip is pulled in the axial direction by the thread, whereby a deformation portion is deformed.