A method for in situ monitoring of a membrane of a membrane separation system comprises measuring a complex impedance of the membrane at a plurality of frequencies to provide an indication of the electrical conduction and electrical polarization properties of the membrane. The membrane based separation system for removing or reducing the concentration of materials carried in a fluid including a separation membrane has a first pair of electrodes separated by the membrane and arranged for measurement of the complex impedance of the membrane at a plurality of frequencies to provide the indication of the membrane properties. There may also be a second pair of electrodes separated by the membrane for injecting the stimulus current such that the injecting and monitoring functions are separated.