An image sensing module utilizes an image sensor to sense objects and a mirror image of the objects in a mirror through a plurality of first light filtering components with a first transmission spectrum and a plurality of second light filtering components with a second transmission spectrum for generating an image. A light filtering module substantially having the first transmission spectrum is disposed in front of the mirror. The image includes a plurality of pixels. Each pixel includes a first sub data and a second sub data. The image sensing module utilizes an image sensing controller to detect real images corresponding to the objects and virtual images correspond to the mirror image of the objects from the image according to the first sub data and the second sub data of the plurality of pixels.