A lens barrel of the present invention comprises: first and second guide shafts arranged parallel to an optical axis X and provided in one of adjacent lens holding frames; first and second guide receiving holes formed in another of the adjacent lens holding frames for allowing the first and second guide shafts to be inserted therethrough and guided by the first and second guide shafts respectively; a first urging means of urging the one and the another of the adjacent lens holding frames along the direction of the optical axis; and a second urging means to rotate another of the adjacent lens holding frames in a circumferential direction of the first guide shaft. The first guide receiving hole is guided by the first guide shaft at two positions that are spaced apart from each other in the direction of the optical axis.