A method of applying acoustic wave to rice seeds for increasing yield comprises the following steps: (1) Process rice seeds by winnowing and/or wet separation; (2) Soak the rice seeds for 10 to 12 hours and pre-process by acoustic wave irradiation in power density from 0.25 to 25 w/L; (3) Use acoustic wave frequency from 10 khz to 2000 khz for acoustic wave processing; (4) Take out the rice seeds processed by the acoustic wave from soaking liquid and keep the rice seeds stationary until they excrete convex white flakes. A device for applying acoustic wave to rice seeds for increasing yield comprises a container (1) for holding rice seeds and soaking liquid, and also a dasher (2); at least one ultrasonic transducer (3) for generating acoustic wave is disposed on sides or bottom of the container.