The present invention relates to an externally operated alpha port for a rapid transfer port (RTP) system such as those found in isolation barrier systems. Means are provided for the actuation by the operator of the alpha port door locking system externally of the isolation barrier to facilitate the locking and unlocking of the alpha port door without requiring gloved entry into the isolation barrier. Means are also provided for opening and closing of the alpha port door with the actuation by the operator of a lever external to the isolation barrier.