Invention Grant
US08951403B2 Detection method for sensor membrane of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes
有权
铕氧化钛传感器膜的检测方法,作为使用PNIPAAm包裹酶的生物传感器的一部分
- Patent Title: Detection method for sensor membrane of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes
- Patent Title (中): 铕氧化钛传感器膜的检测方法,作为使用PNIPAAm包裹酶的生物传感器的一部分
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Application No.: US13478161Application Date: 2012-05-23
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Publication No.: US08951403B2Publication Date: 2015-02-10
- Inventor: Tung-Ming Pan , Chao-Wen Lin , Kung-Yuan Chang , Min-Hsien Wu , Shiao-Wen Tsai
- Applicant: Tung-Ming Pan , Chao-Wen Lin , Kung-Yuan Chang , Min-Hsien Wu , Shiao-Wen Tsai
- Applicant Address: TW Taoyuan
- Assignee: Chang Gung University
- Current Assignee: Chang Gung University
- Current Assignee Address: TW Taoyuan
- Priority: TW100127898A 20110805
- Main IPC: G01N27/327
- IPC: G01N27/327 ; C12Q1/00 ; G01N27/22

Abstract:
A detection method for a sensor membrane formed of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes includes adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60° C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 μl of 98.7 wt % of APS and 50 μl of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 μl of 1×PBS buffer solution, uniformly mixing same, adding 100 μl of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37° C. with the biosensor being an EIS sensor having a sensor membrane formed of EuTixOy; and taking a measurement.
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