Invention Grant
US08951757B2 Method for selective labeling of protein produced by in vitro translation by using a marker from tRNA prepared by using in vitro transcription
有权
通过使用通过使用体外转录制备的来自tRNA的标记物通过体外翻译产生的蛋白质选择性标记的方法
- Patent Title: Method for selective labeling of protein produced by in vitro translation by using a marker from tRNA prepared by using in vitro transcription
- Patent Title (中): 通过使用通过使用体外转录制备的来自tRNA的标记物通过体外翻译产生的蛋白质选择性标记的方法
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Application No.: US12812382Application Date: 2009-01-07
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Publication No.: US08951757B2Publication Date: 2015-02-10
- Inventor: Seongjun Yoon , SooYoun Jun , SangHyeon Kang
- Applicant: Seongjun Yoon , SooYoun Jun , SangHyeon Kang
- Applicant Address: KR
- Assignee: Intron Biotechnology, Inc.
- Current Assignee: Intron Biotechnology, Inc.
- Current Assignee Address: KR
- Agency: Meunier Carlin & Curfman, LLC
- Priority: KR10-2008-0003081 20080110
- International Application: PCT/KR2009/000057 WO 20090107
- International Announcement: WO2009/088209 WO 20090716
- Main IPC: G01N33/68
- IPC: G01N33/68 ; G01N33/533 ; G01N33/532 ; C12P19/34 ; C07H21/02
Abstract:
The present invention relates to a method for the preparation of a labeling agent derived from one type of tRNA prepared by in vitro transcription, which is usable for non-radioactive selective labeling of target proteins produced by in vitro translation with fluorophore or biotin; and a method for selective labeling of target proteins produced by in vitro translation using the labeling agent without labeling pre-existing proteins in the reaction mixture.
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