Invention Grant
- Patent Title: Mammalian expression vector pUHAB
- Patent Title (中): 哺乳动物表达载体pUHAB
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Application No.: US13344905Application Date: 2012-01-06
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Publication No.: US08951790B2Publication Date: 2015-02-10
- Inventor: Deba P. Saha
- Applicant: Deba P. Saha
- Applicant Address: US NJ Rahway
- Assignee: Merck Sharp & Dohme Corp.
- Current Assignee: Merck Sharp & Dohme Corp.
- Current Assignee Address: US NJ Rahway
- Main IPC: C12N15/00
- IPC: C12N15/00 ; C12N15/85 ; C07K16/28 ; C07H21/00 ; C07H21/02 ; C07H21/04 ; C12N5/00 ; C12N5/02 ; C12N5/07 ; C12N15/64 ; C12P21/04 ; C12P21/06
Abstract:
The present invention relates to the construction and utilization of a new mammalian expression vector that contains a unique multiple cloning site (MCS), designated pUHAB. The pUHAB vector comprises a high copy replication origin (ColE1), a drug resistance gene (TK-Hygromycin), and a human cytomegalovirus promoter operably associated with a unique intron (hCMV/intron). Further, pUHAB comprises a selectable marker conferring resistance to kanamycin in bacterial cells, and a phage f1(+) region. pUHAB can be used to transiently or stably express cloned genes when transfected into mammalian cells. The invention also encompasses kits and host cells and cell lines comprising pUHAB, and methods of producing a recombinant protein using pUHAB.
Public/Granted literature
- US20120142056A1 MAMMALIAN EXPRESSION VECTOR pUHAB Public/Granted day:2012-06-07
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