Invention Grant
- Patent Title: Refolding proteins using a chemically controlled redox state
- Patent Title (中): 使用化学控制的氧化还原状态重折叠蛋白质
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Application No.: US12820087Application Date: 2010-06-21
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Publication No.: US08952138B2Publication Date: 2015-02-10
- Inventor: Joseph Edward Shultz , Roger Hart , Ronald Nixon Keener, III
- Applicant: Joseph Edward Shultz , Roger Hart , Ronald Nixon Keener, III
- Applicant Address: US CA Thousand Oaks
- Assignee: Amgen Inc.
- Current Assignee: Amgen Inc.
- Current Assignee Address: US CA Thousand Oaks
- Agent David B. Ran
- Main IPC: C07K1/22
- IPC: C07K1/22 ; C07K1/113
Abstract:
A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
Public/Granted literature
- US20100324269A1 REFOLDING PROTEINS USING A CHEMICALLY CONTROLLED REDOX STATE Public/Granted day:2010-12-23
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