Systems and methods for providing a view of a digital slide image. In an embodiment, a digital slide image file is accessed. The digital slide image file may comprise a plurality of first image planes representing an image of at least a portion of a slide specimen at varying focal depths. Then, a three-dimensional object is constructed from the digital slide image file. The three-dimensional image object comprises a plurality of second image planes that are derived from one or more of the first image planes and may comprise at least one image plane that has been interpolated from one or more of the first image planes. In addition, a two-dimensional and/or three-dimensional view of the three-dimensional object may be generated.