A signaling control method for service establishment based on G.709 is provided in the present invention, including: a control plane obtaining routing information of an end-to-end service to be established, information of a gateway network element passed by end-to-end service routing and a multi stage multiplexing capability selected for the gateway network element, initiating an end-to-end connection establishment process, and sending an end-to-end connection establishment signaling to nodes passed by the end-to-end service routing, wherein, the end-to-end connection establishment signaling contains the multi stage multiplexing capability selected for the gateway network element passed by the end-to-end service routing, and a corresponding multi stage multiplexing capability is configured on the gateway network element. A signaling control system for service establishment based on G.709 is also provided in the present invention. In the method and system, the interconnection between old devices and new devices is better implemented.