A printing apparatus for manufacturing a spacer includes a printing plate and a printing roller. The printing plate has a plurality of receiving recesses formed thereon. The receiving recess has a generally elliptical shape. The receiving recesses are infiltrated with a plurality of beads. The printing roller transfers the beads onto its outer surface by rolling over the receiving recesses, and transfers the beads from its outer surface onto an external substrate. Thus, the spacers may maintain a distance between the first and second substrates stably, so that press characteristics may be enhanced and smear defects occurring when the display panel is pressed may be prevented.