Provided are a method and apparatus for mixing fluids, whereby small amounts of fluids are effectively mixed. An apparatus for mixing fluids includes: a chamber comprising a first region and a second region; a fluid influx channel connected to the first region through which a plurality of fluids flow into the chamber; a turbulent flow generation film interposed between the first region and the second region that includes through-holes through which the fluids are passed to generate turbulent flow in the fluids in the second region to mix the fluids; and a first fluid discharge channel connected to the second region through which the mixed fluids are discharged. Fluids are mixed without additional external devices. Thus, an apparatus for mixing fluids may be miniaturized while effectively mixing fluids.