Invention Grant
- Patent Title: Microfluidic system and method for assembling and for subsequently cultivating, and subsequent analysis of complex cell arrangements
- Patent Title (中): 微流体系统以及用于组装和随后培养以及随后分析复杂细胞装置的方法
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Application No.: US13427733Application Date: 2012-03-22
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Publication No.: US08968543B2Publication Date: 2015-03-03
- Inventor: Martin Stelzle , Brigitte Angres , Massimo Kubon , Julia Schuette , Britta Hagmeyer , Felix Holzner
- Applicant: Martin Stelzle , Brigitte Angres , Massimo Kubon , Julia Schuette , Britta Hagmeyer , Felix Holzner
- Applicant Address: DE Reutlingen
- Assignee: NMI Naturwissenschaftliches und Medizinisches Institut an der Universitaet Tuebingen
- Current Assignee: NMI Naturwissenschaftliches und Medizinisches Institut an der Universitaet Tuebingen
- Current Assignee Address: DE Reutlingen
- Agency: Morrison & Foerster LLP
- Priority: DE102008018170 20080403
- Main IPC: B01D57/02
- IPC: B01D57/02 ; C12M3/06 ; C12M3/00 ; C12M1/42 ; C12M1/26 ; B01L3/00

Abstract:
The present invention provides an apparatus and a method to assemble organotypical tissues which can then be perfused and investigated under preferably physiological conditions. This is achieved by a microfluidic system for assembling and subsequently cultivating complex cell arrangements, comprising: a three-dimensional microstructure in which the cell arrangement is assembled and cultivated, at least two microchannel segments running in the microstructure and defining a flow direction, through which microchannel segments the microstructure can be perfused from outside with a medium, whereby the microchannel segments run approximately parallel or equidistant to one another at least in sections, a wall structure which separates the at least two microchannel segments and in which at least one aperture connecting the at least two microchannel segments is provided, and an electrode arrangement provided in or on the microstructure in order to generate an inhomogeneous electric field in the region of the at least one aperture.
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