Invention Grant
US08969062B2 Mutant proteinase with reduced self-cleavage activity and method of purification
有权
突变蛋白酶具有降低的自我裂解活性和纯化方法
- Patent Title: Mutant proteinase with reduced self-cleavage activity and method of purification
- Patent Title (中): 突变蛋白酶具有降低的自我裂解活性和纯化方法
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Application No.: US13898650Application Date: 2013-05-21
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Publication No.: US08969062B2Publication Date: 2015-03-03
- Inventor: Jennifer A. Doudna , Louise J. Lucast , Robert T. Batey
- Applicant: Yale University
- Applicant Address: US CT New Haven
- Assignee: Yale University
- Current Assignee: Yale University
- Current Assignee Address: US CT New Haven
- Agency: Cooley LLP
- Main IPC: C12N9/50
- IPC: C12N9/50 ; C07H21/04
Abstract:
The present invention provides a mutant 27 kDa NIa proteinase having reduced self-cleavage activity relative to the self-cleavage activity of its wild-type proteinase. The mutant has the same substrate cleavage activity as the wild-type proteinase but is more stable than the wild-type proteinase. The present invention also provides a method of obtaining large quantities of active 27 kDa NIa proteinase for use as a tool for purification of other proteins.
Public/Granted literature
- US20130337503A1 MUTANT PROTEINASE WITH REDUCED SELF-CLEAVAGE ACTIVITY AND METHOD OF PURIFICATION Public/Granted day:2013-12-19
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