Invention Grant
US08969658B2 Method for producing haploid, doubled haploid and/or dihaploid plants by gynogenesis
有权
通过雌激素产生单倍体,双倍体单倍体和/或双倍体植物的方法
- Patent Title: Method for producing haploid, doubled haploid and/or dihaploid plants by gynogenesis
- Patent Title (中): 通过雌激素产生单倍体,双倍体单倍体和/或双倍体植物的方法
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Application No.: US13055210Application Date: 2009-07-21
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Publication No.: US08969658B2Publication Date: 2015-03-03
- Inventor: Muriel Archipiano , Daniele Hosemans , Eric Lionneton , Agnes Vermuelen
- Applicant: Muriel Archipiano , Daniele Hosemans , Eric Lionneton , Agnes Vermuelen
- Applicant Address: FR Portes les Valence
- Assignee: HM. Clause
- Current Assignee: HM. Clause
- Current Assignee Address: FR Portes les Valence
- Agency: McAndrews, Held & Malloy Ltd.
- Priority: FR0854952 20080721
- International Application: PCT/EP2009/059376 WO 20090721
- International Announcement: WO2010/010096 WO 20100128
- Main IPC: A01H1/08
- IPC: A01H1/08 ; A01H1/04 ; A01H4/00 ; C12Q1/68

Abstract:
The invention relates to a method for producing haploid H, doubled haploid HD and/or dihaploid DH plants, the HD and DH being homozygous or essentially homozygous, this method being a method such as those which come under the technique of gynogenesis induced by irradiated pollen. This method comprises a step of irradiating the reproductive material of the male parent at a dose of between 160 and 190 gamma ray and/or a step of selecting the haploid H and/or DH plants by using one or more molecular marker(s). The invention also relates to a method for producing homozygous haploid, doubled haploid and/or dihaploid plants, comprising a step of determining the appropriate irradiation dose(s) for increasing the yields of said plants according to multiple given factors such as the plant species, the genotypes of the male parent and of the female parent, the climatic and weather conditions, the time at which the fruits are harvested, the level of growth of the embryos collected with a view to the culturing thereof, the level of development of the embryos placed in culture. Moreover, the invention concerns the molecular marker(s) used in the selection step and also the haploid embryos and the dihaploid embryos obtained by means of the method of the invention, and the progeny and the seeds of the plants obtained by means of the method of the invention.
Public/Granted literature
- US20110214201A1 Method for Producing Haploid, Doubled Haploid and/or Dihaploid Plants by Gynogenesis Public/Granted day:2011-09-01
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