Method and system for isochronous communication between audio/video (AV) devices. One implementation comprises establishing isochronous connection between a source AV device and a destination AV device. Each AV device includes multiple I/O ports for connecting the AV device to another AV device via a communication link comprising multiple communication lanes. The isochronous connection is established by determining end-to-end temporal and spatial lane availability between the source AV device and the destination AV device to support a target date rate. Communication resources are allocated on the available lanes based on the target date rate for isochronous communication between the source AV device and the destination AV device.