A ladder locking designed intended to prevent access to a tree stand and an attached ladder includes a two-piece interlocking frame, top and bottom hooks to engage rungs of the ladder, and a locking bar. The frames include a mesh covers having a width greater than the tree stand ladder. The frame is secured to the ladder by the top and bottom hooks, which provide opposing hangers hooked over and clamping ladder rungs. A bottom of the frame includes a locking bar which insertingly engages between the two frame sections and secured with a padlock. The frame is placed over a ladder and locked to prevent unwanted persons from ascending the ladder.