Invention Grant
- Patent Title: Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
- Patent Title (中): 用功能域进行序列操作的系统，方法和组合的工程和优化
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Application No.: US14226274Application Date: 2014-03-26
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Publication No.: US08999641B2Publication Date: 2015-04-07
- Inventor: Feng Zhang , Le Cong , Randall Jeffrey Platt , Neville Espi Sanjana
- Applicant: The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
- Applicant Address: US MA Cambridge US MA Cambridge US MA Cambridge
- Assignee: The Broad Institute Inc.,Maassachusetts Institute of Technology,President and Fellows of Harvard College
- Current Assignee: The Broad Institute Inc.,Maassachusetts Institute of Technology,President and Fellows of Harvard College
- Current Assignee Address: US MA Cambridge US MA Cambridge US MA Cambridge
- Agency: Vedder Price P.C.
- Agent Thomas J. Kowalski; Deborah L. Lu
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12N15/85 ; C12N15/63 ; C12N15/01 ; C12N15/86 ; C12N9/14 ; C12N9/22 ; C12N9/52 ; C12N15/00 ; C07H21/02 ; C07H21/04 ; A61K38/43 ; A61K38/46 ; A61K38/47 ; C12N15/10
Abstract:
The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors with additional functional domains. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
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