Invention Grant
US09012159B2 Cell based screening assays for the triglyceride synthesis pathway
有权
甘油三酯合成途径的基于细胞的筛选试验
- Patent Title: Cell based screening assays for the triglyceride synthesis pathway
- Patent Title (中): 甘油三酯合成途径的基于细胞的筛选试验
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Application No.: US12683527Application Date: 2010-01-07
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Publication No.: US09012159B2Publication Date: 2015-04-21
- Inventor: Jian-Shen Qi , Wensheng Lang , Margery A. Connelly
- Applicant: Jian-Shen Qi , Wensheng Lang , Margery A. Connelly
- Applicant Address: US NJ Raritan
- Assignee: Janssen Research & Development, LLC
- Current Assignee: Janssen Research & Development, LLC
- Current Assignee Address: US NJ Raritan
- Agency: Johnson & Johnson
- Agent Bernard F. Plantz
- Main IPC: G01N33/567
- IPC: G01N33/567 ; G01N33/50 ; C12Q1/48 ; G01N33/60 ; G01N33/92
Abstract:
A method for identifying a compound which modulates the activity of acyl-coA: diacylglycerol acyltransferase comprises the steps of contacting a stable isotope labeled fatty acid with cells in either presence or absence of the compound, extracting the cells with isopropyl alcohol, and determining the level of a stable isotope labeled triglyceride in the presence or absence of the compound; wherein a change in the level of the stable isotope labeled triglyceride indicates that the compound modulates the DGAT activity.
Public/Granted literature
- US20100196930A1 CELL BASED ASSAYS FOR THE TRIGLYCERIDE SYNTHESIS PATHWAY Public/Granted day:2010-08-05
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